Lactose Quantification Assay
LACTOSE (C12H22O11), also called milk sugar, is a disaccharide that consists of of b-D-galactose and a/b-D-glucose through a b1-4 glycosidic linkage. Lactose is the major sugar and makes up 2%-8% of milk. Simple, direct and high-throughput assays for lactose determination find wide applications. Amiscience's assay uses specific enzyme-coupled reactions in which lactose is cleaved and the resulting galactose forms a colored product. The fluorescence intensity at 590nm when excited by 530nm is directly proportional to the lactose concentration in the sample measured by FluoroQuik fluorometer.
Specifications
- Sample: Serum, plasma, and other biological samples.
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Assay Performance:
- Sufficient for about 100 tests.
- Linear detection range: 0.04 -1 mM.
- Detection limit: 0.04 mM (1.4 mg/dL, 14 ppm).
- Typical Precision (CV%): <5%.
- Shipping and storage: the kit is shipped on ice. Store Assay Buffer at 4 deg-C and other reagents at -20 deg-C. Shelf life of 12 months after receipt.
