Glycerol Quantification Assay
GLYCEROL [GLYCERIN or GLYCERINE, C3H5(OH)3] is widely used in foods, beverages and pharma-ceutical formulations. It is also a main byproduct of biodiesel production. Simple, direct and automation-ready procedures for measuring glycerol concentrations find wide applications. Amiscience's glycerol assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The fluorescence intensity at ex/em = 530/590nm is directly proportional to glycerol concentration in the sample measured by FluoroQuik fluorometer.
Specifications
- Sample: Serum, plasma, and other biological samples.
-
Assay Performance:
- Sufficient for about 200 tests.
- Sensitive and accurate. Use as little as 10 uL samples.
- Linear detection range: 2 -100 uM, 18 - 920 ug/dL, or 0.18 - 9.20 ppm.
- Simple and convenient. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature.
- Shipping and storage: the kit is shipped on ice. Store Assay Buffer at 4 deg-C and other reagents at -20 deg-C. Shelf life of 12 months after receipt.
