Acetate Quantification Assay
ACETATE is a common anion and fundamental to all forms of life. When bound to coenzyme A, it is central to the metabolism of carbohydrates and fats. It is acid form, acetic acid, is produced and excreted by acetic acid bacteria, such as Acetobacter genus and Clostridium acetobutylicum, which are found universally in foodstuffs, water, and soil. Acetic acid is also a component of the vaginal lubrication of humans and other primates, where it appears to serve as a mild antibacterial agent. Acetic acid is the main component of vinegar, and extensively used in food, dyes, paints, glue and synthetic fibres. Amiscience's assay uses enzyme-coupled reactions to form a colored, fluorescent product. The fluorescence intensity, measured using FluoroQuik handheld fluorometer (ex/em = 530/590nm), is directly proportional to acetate concentration in the sample.
Specifications
- Sample: Serum, plasma, and other biological samples.
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Assay Performance:
- Sufficient for about 100 tests.
- Sensitive and accurate. Use as little as 10 uL samples.
- Linear detection range: 0.1-1.0 mM.
- Shipping and storage: the kit is shipped on ice. Store Assay Buffer at 4 deg-C and other reagents at -20 deg-C. Shelf life of 12 months after receipt.
