Lactose Quantification Assay

LACTOSE (C12H22O11), also called milk sugar, is a disaccharide that consists of of b-D-galactose and a/b-D-glucose through a b1-4 glycosidic linkage. Lactose is the major sugar and makes up 2%-8% of milk. Simple, direct and high-throughput assays for lactose determination find wide applications. BioAssay Systems' assay uses specific enzyme-coupled reactions in which lactose is cleaved and the resulting galactose forms a colored product. The fluorescence intensity at 590nm when excited by 530nm is directly proportional to the lactose concentration in the sample measured by FluoroQuik fluorometer.

Part Number: FLAC-100

Specifications

  • Sample: Serum, plasma, and other biological samples.
  • Assay Performance:
    • Sufficient for about 100 tests.
    • Linear detection range: 0.04 -1 mM.
    • Detection limit: 0.04 mM (1.4 mg/dL, 14 ppm).
    • Typical Precision (CV%): <5%.
  • Shipping and storage: the kit is shipped on ice. Store Assay Buffer at 4 deg-C and other reagents at -20 deg-C. Shelf life of 12 months after receipt.

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