GLYCEROL [GLYCERIN or GLYCERINE, C3H5(OH)3] is widely used in foods, beverages and pharma-ceutical formulations. It is also a main byproduct of biodiesel production. Simple, direct and automation-ready procedures for measuring glycerol concentrations find wide applications. Amiscience's glycerol assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The fluorescence intensity at ex/em = 530/590nm is directly proportional to glycerol concentration in the sample measured by FluoroQuik fluorometer.