AMMONIA (NH3) or its ion form ammonium (NH4+) is an important source of nitrogen for living systems. Ammonia is found in the atmosphere, rainwater, soil, seawater, volcanic areas. It is widely used as fertilizer, cleaner, antimicrobial agents and in fermentation and chemical synthesis. Amiscience's convenient assay uses o-phthalaldehyde reagent to directly measure NH3/ NH4+. The fluorescence intensity measured using FluoroQuik handheld fluorometer at ex/em = 360/460nm is directly proportionate to the NH3 concentration in the sample.